Evaluation Of Bacteriospermia, Antisperm Antibodies And Semen Biochemical Changes As Contributing Factors To Asthenospermia in Sub Fertile Males

 

 

Sleeman Saleh

Khaled Abu AL Teen

Sameer Awadalla

 

 

ABSTRACT
 

The main objective of this project is to focus on some factors in the seminal fluid that are thought to affect sperm motility. These factors include bacteriospermia, antisperm antibodies (ASA) and lipid preoxidation caused by reactive oxygen species (ROS). To achieve this goal, the following steps will be conducted:

1-     Evaluate the prevalence of bacteriospermia and the mean of antisperm antibodies titer in semen samples.

2-     Identify the different types of microorganisms and antisperm antibodies that may be found in semen.

3-     Estimate lipid peroxidation caused by reactive oxygen species (ROS) in the seminal fluid which is known to have severe effect on spermatozoa.

4-     Estimate the levels of the enzymes catalase and superoxide dismutase (SOD) which counteract the toxic effect of ROS and act as antioxidants.

5-     Correlate the above factors with sperm motility in subfertile males.

Results to be obtained from this study should clarify the possible role of bateriospermia and ASA in sperm motility. The results should also illustrate the level of the lipid peroxidation and the level of the antioxidant enzymes superoxide dismutase and catalase ahnd the possible correlation with sperm motility.

 

 

 

 

      * Faculty: Allied Health Sciences/ Medical Laboratory Sciences.

         Specific Specialization: Biochemistry Embryology.