screen mutations of the tumor suppressor gene BRCA1 within three
exons among Jordanian breast cancer females.
Methods: A total
of 135 Jordanian breast cancer females were genetically analyzed
by denaturing gradient electrophoresis (DGGE) for mutations
detection in three BRCA1 exons (2, 11 and 20) between 2000-2002
in Al-Basheer Hospital, Amman, Jordan.
Results: Of the
studied patients fifty had a family history of breast, twenty
eight had a family history of cancer other than breast cancer,
and fifty seven had no family history of any cancer. Five
germline mutations were detected among breast cancer females
with a family history of breast cancers (one in exon 2 and four
mutations in exon 11). Another germline mutation (within exon
11) was detected among breast cancer females with family history
of cancer other than breast cancer, and no mutation was detected
among breast cancer females with no family history of any cancer
or among normal control females.
Screening mutations within exon 2, exon 11 and exon 20 showed
that most screened mutations were within BRCA1 exon 11 among
breast cancer Jordanian families with a family history of breast
and/ or breast-ovarian cancers group.